5 mL of 1 M acetate buffer (pH 4. 190–850 nm <± 1. SpectraMax QuickDrop Micro-Volume Spectrophotometer. 5), L-ascorbic acid (25. Revvity. 2. 304 signal level saturation. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. To. D. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . Intra- and inter-assay coefficients of variation were lower than 8. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. 0. SpectraMax 190 reads 96-well and strip-well plates. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. Crowder, Ziqian Zeng, Alissa N. 1% PEG 8000 at 37°C. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. One plate carrier adapter is provided with the instrument. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). All strains were tested three times and the results averaged. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. The assays contained 0. 25–0. Nitric Oxide Assay. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). The plates were incubated for 4 additional hours at 37°C. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Inter-assay variation was prevented by testing all of the samples on the same day. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. . 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. MMP‑1 inhibition assay. California Lightworks uses only the highest quality LEDs from Osram. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Wavelength Selection. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. SpectraMax Microplate Readers. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 0% and ± 0. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. Homeostatic model assessment for insulin. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. Microplate Readers. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Novak, Nathan J. We also offer depot Repair Service from our base of operations in Southern California (San Diego). A. Warranty. Money Saved: $340. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Donor filter: 616/10 nm. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. , Sunnyvale, CA). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. gp70 blocking. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Molecular Devices’ PathCheck® sensor technology. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. Microplate Spectrophotometers. Alkaline phosphatase (ALP) activity. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. This reader only allows high performance HTRF measurement when assays are run in. Data were expressed as the mean LPS-treated cells (100%) vs. These robust readers have been placed in labs from Antarctica to the. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Immunofluorescence and Aggregation Analysis. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. chen@case. Please see photos. 313 reference gain check fail. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. Awareness Technology, Inc. All data were normalized to experimental day 0. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. Molecular Devices SPECTRAmax. more on but is otherwise untested and sold AS-IS. The contents of the. 190–850 nm <± 1. Number of flashes. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 04. Molecular Devices. Quick View. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. SpectraMax Multi-Mode Microplate Readers. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. Cell proliferation was measured using the MTT method. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Stat Fax 4700 Microstrip Reader. 5. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. , Sunnyvale, CA). Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. using SpectraMax i3 (Molecular devices, San Jose, USA). ScanLater Western Blot Detection Cartridge. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 3. 94 g calcium chloride dihydrate (both. Details. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). 001/min at 425 nm. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. Description. Microscopy. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. 2. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. , Sunnyvale, CA, USA) at 595 nm. TGFα release was calculated as described in the Results. The ABS reader ranges from. SpectraMax Microplate Readers. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. edu Contact Phone: 216-368-8962. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). The absorbances were being measured at 515 nm. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. The values were corrected for nonspecific background by subtracting the OD 650. SpectraMax® Microplate Readers and SoftMax® Pro Software. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. (1988) and recorded at 412 nm. Lactate Dehydrogenase (LDH) Release. Oxidative stress induction by H 2 O. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. The light features a slim profile to prevent. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Immunofluorescence and Aggregation Analysis. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 5 - 2. , Sunnyvale, CA). A. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 0% and ± 0. Manager: Yinghua Chen, PhD Contact Email: yinghua. SpectraMAX 340 plate reader (Molecular Devices). 2 0. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. One plate carrier adapter is provided with the instrument. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. 10× trypsin 0. This assay measures the two major sugars in wine, glucose and fructose. Software (SoftMax Pro ® software or comparable plate reader software). Details. g. Cell culture 3T3 fibroblasts and RAW 264. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 2. edu Contact Phone: 216-368-8962. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Molecular Devices SpectraMAX 340 Microplate Reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. They are for reference only. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). 2. Rate of fibrinolysis. 3 0. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The reaction mixture was incubated for 5 min. SpectraMax 340 from Molecular Devices). Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 30 µs. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. One plate carrier adapter is provided with the instrument. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. ). The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. edu Contact Phone: 216-368-8962. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. 0200-7027. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. 306 plate air cal. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. 00. TDP-43 stress granular cells were treated with 70% ice-cold. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 6. Molecular Devices Corporation 1311. SpectraMax Injector Cartridge with SmartInject™ Technology. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). 5. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). Immunofluorescence and Aggregation Analysis. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). , Sunnyvale, CA, USA) at 595 nm. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. 3: The cuvette chamber. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. ATI/240, 250. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Clotting times (mean ± SD) for rSimukunin and rSV. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. View the Molecular Devices website. View the Molecular Devices website. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . The chamber contains. Molecular Devices SpectraMax M5e Microplate Reader. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Microplate Readers. 5. AoSMCs were seeded into 6-well culture plates. Manager: Yinghua Chen, PhD Contact Email: yinghua. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. 0. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Amino Acid Analysis. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Quick View. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). 1 mL of acetylacetone. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. Anti-proteolytic activity. 315 can't find zero order. Includes: Computer Loaded with Softmax Pro 5. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. 340/70 nm. Call 1-800-745-2710. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. D2. In vivo anti-malarial assay in mice. ): 8. Donor: 620 nm. When necessary, samples were. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. One plate carrier adapter is provided with the instrument. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. 30. PerkinElmer. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Products : SpectraMax Plus 384 reader,. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. SoftMax Pro. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. 6 (h) x 22. TDP-43 stress granular cells were treated with 70% ice-cold. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 7. Temperature Range: Ambient + 4 °C up to 45 °C. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. 2. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. 190-1000 nm, tunable in 1 nm increments. The first time you replace an OEM lamp you must use the N-34-MLA. MOLECULAR DEVICE M5. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Thin layer chromatography (TLC) was. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). Absorbance was recorded at 540 nm using SpectraMAX 340 reader. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Labsystems Luminoskan RT Microplate Reader. albicans pre. used. Customer Transformation Hub. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). These lab workhorses are used in drug discovery, research, bioassay validation, and. P. 024). The loading equaled the mass of BSA per mass of particles. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. Microplate Readers. 2 g/L solution; dilute 1:10 in HBSS. Read one sample or up to 384. , Sunnyvale, Calif. Both readers feature an 8-channel read head for fast reads and. 8. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. reader (SpectraMax 340; Molecular devices, LLc). Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). , Sunnyvale CA). The plates were incubated for 4 additional hours at 37°C. 6. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. A 0. 2 - 3. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. , Oxon, UK). Each experiment was performed in duplicate and repeated at. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete.